【摘要】 目的:研究氟烷、异氟烷和恩氟烷对缺血再灌注心肌功能、代谢自由基的影响。方法:SD大鼠104只,随机数字表法分为13小组,每组8只。采用Lan-gendorff离体大鼠心脏模型。按给药方式又分为对照、氟烷、恩氟烷、异氟烷4大组。①对照组(含4小组):平衡15min组,平衡后续灌15min组,平衡续灌后缺血10min组,平衡续灌缺血25min后复灌30min组。②氟烷组(含3小组):平衡15min后,灌注含1.5肺泡气最低有效浓度(minimalalveolarconcentration,MAC)氟烷灌注液15min组,平衡续灌后缺血10min组,平衡续灌缺血25min复灌含1.5MAC氟烷的灌注液30min组。③恩氟烷和异氟烷组,各包括3小组,情况同氟烷组。各组记录平衡后,给药(或续灌15min)复灌30min左心室收缩压、左心室舒张末期压(leftventriculardiastolicpressure,LVEDP)、左心室发展压、左心室压力升高或降低最大速率、心率、冠状动脉流量(coronaryflow,CF)。实验结束后测定心肌超氧化物歧化酶(superoxidedismutase,SOD)活性、心肌丙二醛含量、高能磷酸盐(ATP)含量。结果:①恩氟烷、异氟烷具有明显扩张冠状动脉的作用。恩氟烷能促进缺血再灌注心肌冠脉流量的恢复。②各用药组明显降低左心室发展压、左心室压力升高速率,升高LVEDP(P均<0.05);缺血再灌注后,氟烷、恩氟烷、异氟烷组的?更多还原

【Abstract】 AIM:To investigate the effects of halothane,isoflurane and enflurane on myocardial function,metabolism and oxygen free radical in ischemia reperfusion(IR) rats. METHODS:Totally 104 SD rats were randomly divided into 13 groups(8 rats in each group) by means of random number table.The 13 groups were regrouped into control group,halothane group,enflurane group and isoflurane group according to the manner of administration.①The control group consisted of four subgroups:15 minute equilibrium group,15 minute perfusion after equilibrium group,10 minute ischemia after equilibrium and perfusion group and 25 minute reperfusion after equilibrium,perfusion and ischemia group.②The halothane group consisted of 3 subgroups:15 minute perfusion with 1.5 minimal alveolar concentration(MAC) halothane group after 15 minute equilibrium,10 minute ischemia after equilibrium and perfusion group and 30 minute reperfusion with 1.5MAC halothane after equilibrium,perfusion and 25 minute ischemia group.③The enflurane and isoflurane groups consisted of 3 groups administrated with the enflurane and isoflurane instead of halothane respectively.After equilibrium followed by administration or 15 perfusion and 30 perfusion,left ventricular systolic pressure(LVSP),left ventricular diastolic pressure(LVEDP),left ventricular developed pressure(LVDP),?dp/dt and -dp/dt,heart rate and coronary flow(CF) were monitored.The contents of myocardial adenosine triphosphate(ATP) and malondialdehyde(MDA) and the activity of superoxide dismutase(SOD) were determined after treatment. RESULTS:①Enflurane and isoflurane could obviously dilate coronary artery.Enflurane could accelerate the recovery of CF.②The LVDP and ?dp/dt were significantly decreased,while the LVEDP was significantly increased in all the treatment groups(all P< 0.05).After IR in the halothane,enflurane and isoflurane groups,the LVDPs recovered to 57%,62%and 59%of control value respectively(all P< 0.01),the ?dp/dt results were recovered to 56%,67%and 74%of baseline respectively(all P< 0.01),significantly different from the LVDP(27%) and ?dp/dt(13%) respectively in the control group(both P< 0.01).The myocardial ATP contents were increased in all the treatment groups.③The myocardial ATP content was decreased slowly after ischemia,while recovered rapidly after reperfusion.④The activity of SOD in the treatment groups was significantly higher than that in the control group,while myocardial MDA content in the treatment groups was significantly lower than that in the control group.CONCLUSION:Three kinds of volatile anesthetics inhibit myocardial systolic function in normal myocardium.After IR,the volatile anesthetics improves the recovery of function and metabolism, and increases CF and the activity of SOD,indicating that it may be one of the mechanisms of volatile anesthetics against IR injury.更多还原