【摘要】 目的:检测胃肠道黏膜相关淋巴组织淋巴瘤(mucosa-associatedlymphoidtissuelymphoma,MALToma)中3号染色体(chromosome3,C3)三体的发生率,并探讨此变异与该肿瘤的发生关系及在临床治疗中的应用。方法:根据新WHO分类标准,选择33例诊断为胃肠道MALToma的标本,对实验成功的27例进行分型,16例为经典的MALToma,临床为惰性。11例在MALToma基础上有大细胞转化,为转化的MALToma,临床为侵袭性。选用生物素标记的染色体特异的着丝粒探针,采用染色体原位杂交方法,以16号染色体探针作为技术参照组,以胃肠道慢性炎症作为实验对照组,检测肿瘤细胞中3号染色体的拷贝数。结果:在16例惰性MALToma标本中8例为C3三体,8例为正常的C3二倍体;11例转化的MALToma标本中8例为C3三体,3例为正常的C3三体。C3三体在胃肠道MALToma中的发生率达50%,在转化的MALToma中发生率达72.7%,所有的病例都未进行临床系统的抗HP治疗。结论:C3三体在胃肠道黏膜MALToma中发生率较高;该高发生率与肿瘤的发生或演进可能有一定的相关性;并对协助临床诊断及治疗方案的选择具有价值,对患者的临床预后有一定的提示作用。更多还原

【Abstract】 AIM:To determine the incidence of trisomy of chromosome 3(C3)(trisomy 3) in gastrointestinal mucosa associated lymphoid tissue lymphoma(MALToma),and to find the relationship between trisomy 3 and the occurrence of entity as well as the application of trisomy 3 in clinical treatment. METHODS:Specimens of totally 33 cases of gastrointestinal MALToma were selected pathologically according to the new standard of WHO classification.Twenty seven of the 33 cases successfully studied were typed:16 cases of classical MALTomas,which were low grade MALToma and indolent in clinic and 11 cases of MALTomas with large cells transformed,which were transformed MALToma and aggressive in clinic.The centromeric DNA probes specific for C3 labeled with biotin were used,and the technique of chromosome in situ hybridization(CISH) was applied in this study.Chromosome 16 probe as the technique control group,and gastrointestinal chronic inflammation as the experiment control group were adopted to detect C3 copies in tumor cells. RESULTS:In low grade MALToma group,8 cases were trisomy 3(50%,8/16),8 cases were normal disome.In high grade MALToma group,8 cases were trisomy 3(72.7%,8/11),while 3 cases were normal trisomy.All the cases were not treated with anti helicobacter pylori. CONCLUSION:The incidence of trisomy 3 in the gastrointestinal MALToma is very high,indicating that it may associated with the pathogenesis or development of MALToma in a certain extent.Trisomy 3 is available to the diagnosis and the treatment of MALToma,and has some predicts for the clinical prognosis.更多还原