【摘要】 应用金霉素(CTC)荧光检测、精子穿卵试验和项体酶β-D-半乳糖苷酶活力测定等方法检测冻存对大鼠精子顶体的影响。CTC荧光反应显示,与未冻存精子比较,冻存后精子顶体反应的类型发生改变,AR型(发生了顶体反应)精子比例明显下降,冻存前为68.6％,冻存后为13.4.％,但是获能精子的比例未发生明显变化(92.6％对90.8％)。与未冻存的精子相比,冻存组精子的穿卵试验的受精指数下降(23.4±7.02对10.2±3.95),而且冻存后精子顶体酶——β-D-半乳糖苷酶活力明显下降(9.39±1.98对4.50±1.40)。结果表明冻存后精子的顶体功能受到较明显的破坏。实验结果为今后针对性地改进大鼠精子的冻存方法,改善大鼠精子的冻存保护剂提供实验和机制上的依据。更多还原

【Abstract】 With the best cryopreservation protocol and the cryoprotectant for rat spermatozoas we could found, the effect of cryopreservation on the acrosome of the spermatozoas from SHR were studied. The effect of cryopreservation on the acrosomal reaction of SHR spermatozoa is determined by the ways such as chlortetracy-cline (CTC) staining, sperm penetration of zona-free egg assay, and β-D-galactosidase activity analysis. The acrosomal reaction rate of frozen rat spermatozoas (13.4%) was significantly lower than that of unfrozen (68.6%). Compared to the spermatozoas unfrozen, the fertility index of frozen spermatozoas was significantly reduced (23.4±7.02 and 10.2±3.95, respectively); as well as that of β-D-galactosidase activity(9.39±1.98 and 4.50±1.40, respectively). The present results indicated that cryopreservation impaired the acrosome of rat spermatozoas.更多还原