【摘要】 针对QB1502-92标准测定方法测定高活力果胶酶、固定化果胶酶活力存在的实验误差大,重现性及灵敏度差的缺陷,提出了利用3,5-二硝基水杨酸与醛糖共热产生棕红色的氨基化合物,在一定范围内还原糖的量和含有呈色氨基化合物的反应液颜色深浅成正比的原理,在540nm下测其吸光度,从而计算出果胶酶活力的测定方法,并对测定步骤、影响因素及测定中可能出现的问题进行了试验研究。结果表明,该方法简便、准确、重现性高,适合高活力果胶酶和固定化果胶酶活力的测定。更多还原

【Abstract】 A method for determinating the activities of pectinase was proposed in this paper. It can produce the red-brown amino compounds when 3,5-dinitrosalicylic acid and aldehyde were heated together. The amino compounds had maximum absorbency at 540nm. In certain range, there was a good linear relationship between the amount of reducing sugar and the color of the reaction mixture. The activities of pectinase can be calculated by determiniting the absorbency at 540nm. The steps, the factors and other problems that may occur in the experiment were studied here. The result showed that the method was more simple, accurate and it also had good reproducibility. It was more efficient than QB1502 and other methods, especially in determinating the activities of immobilized pectinase and high activity pectinase.更多还原