【摘要】 目的　采用近临界水降解壳聚糖得到一系列具有生物活性的寡糖,并研究其最佳工艺条件。方法　壳聚糖在自制的超临界流体反应装置中降解,通过红外光谱确定产物的结构,用硫酸 蒽酮法测定寡糖含量,并用薄层色谱测定寡糖聚合度的分布。结果　在近临界水中,壳聚糖能够短时间内降解成小分子产物,寡糖产率随着压力的增大而增大,随着温度升高及时间的增加,寡糖的产率先增后减。结论　壳聚糖在近临界水中降解的适宜压力为25MPa,温度为250℃,时间为5min。更多还原

【Abstract】 Aim Chitosan was degraded in the near-critical water into a series of products that were bioactive, and the distributing orderliness of oligosaccharides was studied.Methods Chitosan was degraded in the self-made supercritical fluid reactor.The structure of the products was determined by IR, the content of oligosaccharides was mensurated by the method of sulphuricacid-anthrone,and the distributing orderliness of products was mensurated by TLC.Results Chitosan can be degraded into low molecular weight products in near-critical water in a short time, the content of oligosaccharides increased with the increasing of pressure, but increased first and reduced finally with the raising of temperature and the extending of time.Conclusion 25 MPa,250℃ and 5 min are the condign technical conditions for the degradation of chitosan.更多还原