【摘要】 目的　研究熊去氧胆酸 (UDCA)对免疫性大鼠肝纤维化的影响 ,并探讨其作用机制。方法　 3 6只SD大鼠随机分为 3组。 2 4只大鼠腹腔内注射猪血清 (每周 2次 ,共 8周 )诱导建立肝纤维化模型 ;其中 12只予以UDCA[15mg/(kg·d]干预 (UDCA组 ) :余 12只为模型组。对照组 12只大鼠未予任何处理。 8周后处死动物。观察肝组织纤维化程度、胶原表达及血清透明质酸 (HA) ;羟脯氨酸 (Hyp)水平的变化 ;免疫组化染色观察α 平滑肌肌动蛋白 (α SMA)、转化生长因子 β1(TGF β1)及核因子κB(NFκBp65 )表达的情况 ;各组结果分别进行比较。结果　模型组大鼠肝内胶原面积明显高于对照组 ( 11 1± 2 0 9vs 0 73± 0 15 ,P <0 0 5 ) ,血清HA、Hyp及α SMA、TGF β1,NFκBp65在肝内的表达亦显著增高 (P <0 0 5 )。与模型组相比较 ,UDCA组肝内胶原面积无明显变化 ( 9 49±1 3 1vs 11 1± 2 0 9,P >0 0 5 ) ,其余指标在两组间均无显著性差异 (P >0 0 5 )。结论　一般剂量的UDCA对大鼠免疫性肝纤维化无显著改善作用更多还原

【Abstract】 Objective To assess the effects of ursodeoxycholic acid （UDCA） in preventing immunodamaged hepatic fibrosis induced by pig serum in rats,and to explore its mechanisms of anti-fibrosis.Methods Thirty-six Sprague Dawley rats were randomly divided into three equal groups. Hepatic fibrotic models were established by injection of pig serum into the peritoneal cavity twice/week for 8 weeks in two groups. One of them was treated with UDCA 15mg/（kg·d） for 8 weeks（UDCA group）, the other was regarded as model group. The remaining group was without any treatment（control group）. All rats were sacrificed 8 weeks later. Liver fibrosis was assessed directly by hepatic morphometric analysis, the levels of hyaluronic acid （HA）, hydroxyproline（Hyp） were evaluated,and the expression of α-smooth muscle actin （α-SMA）,transforming growth factor-beta 1（TGF-β₁）,nuclear factor-NF κ B （NF κ B p65） in liver tissue were detected by immunohistochemical techniques. The data from UDCA group was compared with that from model group and control group respectively.Results The area of collagen of liver tissue in model group was much higher than that in control group（11.1±2.09 vs. 0.73±0.15, P<0.05）, and so did the serum level of HA, Hyp and the expression of α-SMA, TGF-β₁ and NF κ Bp65 （P<0.05）. Compared with model group,the area of collagen in UDCA group was not markedly decreased （9.49+1.31 vs. 11.1+2.09, P>0.05）.The same results were obtained from other indexes（P>0.05）.Conclusions UDCA with ordinary dosage may not significantly delay the progression of pig serum-induced liver fibrosis in rats.更多还原