【摘要】 目的　探索着床窗口小鼠子宫内膜上皮细胞的原代培养方法 ,并对培养细胞进行形态学检测。方法　取着床窗口 (妊娠d 4 )小鼠双侧子宫角 ,用胰蛋白酶消化和机械削刮法相结合分离上皮细胞 ,经高密度接种及添加EGF培养后 ,通过光镜和透射电镜观察其形态学特征。结果　接种 2 4h后大部分细胞已贴壁 ,培养 4d后细胞排列紧密、长成上皮细胞集落 ,电镜显示细胞为分泌上皮细胞 ,细胞纯化率在 90 %以上。结论　采用上述方法可成功地分离、原代培养着床窗口小鼠子宫内膜上皮细胞。更多还原

【Abstract】 Objective: To discuss the primary culture and morphologic detection of the isolated endometrium epithelial cells from mice during the implantation window. Methods: The two uteri horns separated from mice during the implantation window(or the 4th day of pregnancy) were incubated with trypsin, then the epithelial cells were gently scraped away. After dense culturing and adding EGF, the cultured cells were observed by light microscopy and transmission electron microscopy(TEM). Results: Most cells became adherent after 24 hours of culture and formed tightly packed cell-aggregates after 4 days. Observation with TEM showed that these cells were glandular epithelium and their purity were above 90%. Conclusion: Endometrium epithelial cells of mice during the implantation window were successfully isolated and primarily cultured in this experiment.更多还原