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丛枝菌根化大青杨苗木根际微域环境的研究
The rhizospheric niche of seedlings of Populus ussruiensis colonized by arbuscular mycorrhizal (AM) fungi
【摘要】 对接种AM菌根真菌的大青杨苗木根际微区进行系统研究,发现AM菌根真菌对宿主根际微域环境产生了重要影响。试验中选用的Glomus mosseae、G. intraradices、G. sinuosa、G. versiforme等4种AM菌根真菌都与盆栽大青杨苗木形成了菌根复合体。其中G. mosseae、G. intraradices侵染效果最好,侵染率分别是64.4%、67.4%。受两种菌侵染的苗木的生物量分别是对照处理的2.59、2.13倍。AM菌根对根际土壤微生物种群数量没有产生影响,但使根面、根系上的细菌、放线菌、固氮菌的数量显著增加。AM菌根增加了根际土壤磷酸酶、脲酶、蛋白酶的活性,各种酶活性增加量与苗木菌根侵染率呈显著相关。AM菌根使根际pH值降低,与菌根侵染率呈显著负相关。接种苗木的根际土壤中,可直接被植物吸收利用的N、P元素出现富集现象,与菌根侵染率呈极显著相关。
【Abstract】 Through systemic study on the rhizospheric niche of seedlings inoculated with arbuscular mycorrhizal (AM) fungi, we found that the niche of the host-plant was affected vitally by AM fungi. The selected four AM fungi Glomus mosseae, G. intraradices, G. sinuosa, G. versiforme infected the potted seedlings of Populus ussruiensis and formed mycorrhizal compound with the host plant. Glomus mosseae and G. intraradices have better infection rate than the others. Their infection rates were 64.4%, 67.4%, respectively. The biomass of seedlings infected by the two AM fungi were 2.59, 2.13 times of control, respectively. AM fungi don抰 affect on microbial populations in rhizospheric soil, but increase the number of bacteria, actinomycetes and azotobacter in the root surface and root surround area. AM fungi accelerated the activities of phosphatase, urease, and proteinase in soil rhizosphere, and the activities of these enzymes were positively correlated with the infection rate of AM fungi. AM fungi decreased the pH value of rhizosphere, but the pH value is correlated with the infection rate of AM fungi negatively. The nutrient gathering of available N and P in the rhizosphere of the seedlings inoculated with AM fungi was found, and it was correlated with the infection rate of AM fungi remarkably.
【Key words】 AM fungi; Populus ussruiensis; rhizospheric soil; microbe; soil enzyme activity; pH; soil nutrient;
- 【文献出处】 生态环境 , 编辑部邮箱 ,2004年02期
- 【分类号】S154.36
- 【被引频次】78
- 【下载频次】475