【摘要】 目的　研究地塞米松 (Dex)对星形胶质细胞细胞周期的影响。方法　纯化培养的大鼠大脑皮质星形胶质细胞分为 4组 ,分别加入不同浓度的Dex (0、 10 -5、 10 -4、 10 -3 mol/L)培养 18h后 ,经流式细胞仪检测细胞凋亡及细胞周期。结果　 10 -3 mol/L的Dex诱发了星形胶质细胞的凋亡。 4个组的细胞周期各时相的细胞指数 :G0 /G1期依次为86 2 9± 0 2 8、 88 0 6± 0 0 3、 91 2 2± 1 14和 83 10± 2 39,前 3组随浓度增加而增加 ,第 4组例外 ;S期依次为 2 6 7±0 14、 3 5 9± 0 30、 2 97± 0 4 4和 6 0 6± 0 2 5 ,前 3组间无显著差异 ,第 4组明显增加 ;G2 /M期依次为 11 0 3± 0 5 1、8 35± 0 75、 5 81± 0 4 9和 10 84± 1 2 9,前 3组随浓度增加而减少 ,第 4组例外。S期细胞指数与G2 /M期细胞指数之比 ,分别为 1∶4 13、 1∶2 33、 1∶1 96和 1∶1 79,后 3组与第 1组有明显的差异。结论　Dex对星形胶质细胞的细胞周期进程有明显的抑制。治疗剂量的Dex抑制星形胶质细胞由S期向G2 期的转换 ,10 -4mol/L以下的浓度同时抑制其由G1期向S期的转换。大剂量的Dex可引起星形胶质细胞凋亡。更多还原

【Abstract】 Objective To Study the effect of dexamethasone (Dex) on the cel l cycle in astrocyte.Methods Astrocytes cultured purely from newborn rats,cerebral cortex were divided into 4 groups each being treated with different concentratio ns (0, 10 -5, 10 -4, 10 -3 mol/L) of Dex. After cultured for 1 8 h, the apoptosis and the cell cycle of the astrocytes in 4 groups were examine d by the flow cytometer. Results 10 -3 mol/L Dex could induce apoptosis of astrocy tes. The cell indexes in different phases in 4 groups were as follows: 86.29±0 .28, 88.06±0.03, 91.22±1.14 and 83.10±2.39 respectively in G 0/G 1 p hase; 2.67±0.14, 3.59±0.30, 2.97±0.44 and 6.06±0.25 respectively in S phase; 11.03±0.51, 8.35±0.75, 5.81±0.49 and 10.84±1.29 respectivel y in G 2/M phase. The ratio of cell index between S and G 2/M in 4 groups was 1∶4.13, 1∶2.33, 1∶1.96 and 1∶1.79 respectively. There was remarkable dif ference between 2—4 groups and 1st group. Conclusion The cell cycle of astrocytes was blocked markedly by Dex. The Dex (10 -5, 10 -4, 10 -3 mol/L) inhibited the conver sion of cells from S into G 2. Dex of 10 -5 mol/L and 10 -4 mol/L s uppressed the conversion of cells from G 1 into S simultaneously. Dex of 10 -3 mol/L could induce apoptosis of astrocytes.更多还原