【摘要】 目的 :探索口腔黏膜鳞状细胞癌端粒酶活性与增殖细胞核抗原 (PCNA)表达的关系。方法 :应用TRAP PCR ELISA方法及免疫组化SP法对 68例口腔黏膜鳞状细胞癌组织、3 4例癌旁上皮组织和 12例正常口腔黏膜组织进行检测。结果 :口腔黏膜鳞状细胞癌组织中端粒酶表达阳性率为 67.65 % ( 4 6/ 68) ;癌旁组织中端粒酶表达率为 8.82 % ( 3 / 3 4) ;正常口腔黏膜组织中无端粒酶活性表达。口腔黏膜鳞状细胞癌组织端粒酶活性表达与临床病理分级有关 ;端粒酶活性阳性的 46例癌组织和 3例癌旁上皮异常增生组中PCNA阳性细胞密度为 ( 165 .3 1± 1.82 )个 /mm2 ,端粒酶阴性的 2 2例癌组织和 10例癌旁上皮异常增生组中PCNA阳性细胞密度为 ( 96.77± 1.74)个 /mm2 。端粒酶活性阳性组与阴性组之间PCNA阳性细胞密度差异有统计学意义 (P <0 .0 1)。结论 :端粒酶活性与口腔黏膜鳞状细胞癌发生、发展密切相关 ;端粒酶活性与PCNA阳性表达有关更多还原

【Abstract】 Objective:To study the relationship between telomerase activity and the expression of proliferating cell nuclear antigen(PCNA) in oral squamous cell carcinoma (OSCC). Methods:Telomerase activity and expression of PCNA in 68 cases of OSCC,34 cases of epithelium immediately adjacent to carcinomas and 12 cases of normal oral mucosa were detected by TRAP-PCR-ELISA and immunohistochemical SP method. Results:The positive rate of telomerase in OSCC, the epithelium immediately adjacent to carcinomas and the normal oral mucosa was 67.65%(46/68), 8.82%(3/34) and 0(0/12) respectively. Telomerase activity in OSCC was related to the grade of the clinicopathology(P<0.001). Positive cell density of PCNA in telomerase positive and negative samples was (165.31±1.82) /mm 2 and (96.77±1.74) /mm 2 respectively (P< 0.01). Conclusion:The telomerase activity might contribute to the progression of OSCC. The activity of telomerase is positively related to the expression of PCNA.更多还原