【摘要】 目的 :建立一种简便、快捷的β淀粉样蛋白的电泳分离方法。 方法 :比较以往报道的各种低分子量蛋白的电泳分离方法 ,对电泳缓冲液系统、分离胶和浓缩胶的浓度、电泳程序等进行了优化。结果 :用改进的TwophasesTris -Trincine -SDS -PAGE可以简单、快速、清地分离 β淀粉样蛋白。结论 :新建立的TwophasesTris-Trincine -SDS-PAGE是一种较好地分离β淀粉样蛋白和其它超低分子量蛋白或多肽的电泳方法更多还原

【Abstract】 Objective:To set up a simple and rapid SDS-PAGE system to separate β-amyloid protein.Methods:Based on several reported SDS-PAGE systems for low molecular weight protein,the buffer systems,concentrations of separating gel and stacking gel,electrophoresis procedures were optimized.Results:β-amyloid protein was separated simply,rapidly and clearly by the improved two phases Tris-Trincine-SDS-PAGE.Conclusion:Two phases Tris-Trincine-SDS-PAGE is a good SDS-PAGE system for ultro-low molecular weight protein separation.更多还原