【摘要】 目的是通过研究微量元素对乙酰乳酸脱羧酶的影响 ,来提高乙酰乳酸脱羧酶的活力。在实验室阶段 ,考察了多种微量元素对乙酰乳酸脱羧酶发酵酶活力的影响 ,通过单因子实验确定出微量元素的最佳浓度 ,并通过正交试验来摸索最佳的组合。验证试验结果表明 :四种微量元素在最佳浓度分别镁 0 .0 0 2 5g L、锰 0 .0 0 0 2 5g L、钼 0 .0 0 2 5g L、钡 0 .0 0 0 5g L的组合下 ,可使酶的激活率达 199%以上。更多还原

【Abstract】 The purpose of paper is to improve the power of ALDC by researching on trace element effect on the fermentation of ALDC,in the laboratory,studied the effect of various trace element on ALDC production of Bacillus subtilis and search optimization concentration,at last ascertain the proportion of trance element were ascertained by the experiment of orthogonal.The results showed:the optimal compounding was magnesium 0.0025g/L,manganese 0.00025g/L,molybdenum 0.0025g/L,barium 0.0005g/L and the power of ALDC reach 199% at optimization concentration.更多还原