【摘要】 放线细菌的 2 3SrRNA基因序列具有较好的保守性和相对的可变性 ,被认为是快速筛选放线细菌的合适部位。能否快速有效地扩增特异性区间 ,聚合酶链反应 (PCR)扩增前的模板制备质量是关键 ,故比较了基因组DNA作为PCR模板的 5种制备方法。旨在寻找准确、快速、简便、经济的放线细菌筛选技术 ,为普通和极端环境放线细菌资源的研究和开发利用创造条件。更多还原

【Abstract】 The 23S rRNA gene of Actinobacteria is always considered as a suitable location for fast screening Actinobacteria from bacteria because it is conserved and variable.It is critical to get high quality samples before PCR amplification.Therefore five genomic DNA extraction methods were compared with each other.The aim of this study is to search one new method, which could be used as accurately,fast,easily and economically screening Actinobacteria,providing a platform for exploration and utilization Actinobacteria resources under normal and extreme environments.更多还原