【摘要】 综述了抗氧化保健食品清除O2·、·OH、H2O2和DPPH·自由基作用的体外实验方法和原理。检测保健食品清除自由基的体外实验方法有多种,一般采用化学方法在体系中产生自由基,通过测定自由基与体系中的化学物质或离体组织成分反应所产生的颜色变化、发光现象等间接测定自由基含量或根据氧张力的变化等直接测定自由基含量。当体系中加入有清除自由基作用的保健食品功效成分后,体系中的自由基含量会减少,由此确定保健食品清除自由基能力的高低。体外实验的特点是快速、灵敏,可对保健食品清除自由基作用进行初步分析评价,适用于筛选抗氧化保健食品功能材料。更多还原

【Abstract】 A summary on the in vitro experimental methods and principles for the elimination of O2·、·OH、H2O2 andDPPH· free radicals by health functional foods .The chemical methods in witch system the free radicals can be produced fordetecting the elimination effect on the free radicals were introduced. In these methods, by the production of color changes or lightemission phenomenon can be identified indirectly the free radical content or by detecting the oxygen tension changes to identifythe free radical content directly. The experiments showed, adding the health functional food having the elimination effect on thefree radicals to the detecting system, the free radicals in the system minimized, according to this, the quality of the healthfunctional food for the elimination free radical effect can be ascertained, The in vitro detection is fast and sensitive, it’s good forpreliminary screening the eliminating free radical health functional food.更多还原