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粟酒裂殖酵母中辅酶Q10的提取和测定方法
Extraction and Mensuration of CoQ_ (10) in Schizosaccharomyces promb
【摘要】 通过对高效液相色谱法测定辅酶Q10 与紫外分光光度法测定辅酶Q10 的比较 ,确定了两者之间的相关性 ,确定利用紫外分光光度法代替高效液相色谱法测定辅酶Q10 ,从而简化辅酶Q10 的测定方法 ;通过对醇碱皂化法和碱皂化法提取辅酶Q10 的比较 ,确定利用碱皂化法提取发酵液中的辅酶Q10 ,并进一步优化了碱皂化法的提取工艺。
【Abstract】 The extraction and mensuration of CoQ 10 from Schizosaccharomyces promb 2.1794 were studies in this paper. Through experiments we established the optimal extraction techniques. HPLC and UV method were used to mensurate the content of CoQ 10 in the culture. By comparing the methods of HPLC and UV, it was determined that UV could replace HPLC in measuring the content of CoQ 10 to a certain extent.
【关键词】 辅酶Q10;
测定;
醇碱皂化法;
碱皂化法;
【Key words】 coenzyme Q 10; mensuration; cell fragmentation; extraction;
【Key words】 coenzyme Q 10; mensuration; cell fragmentation; extraction;
- 【文献出处】 食品与发酵工业 ,Food and Fermentation Industries , 编辑部邮箱 ,2004年11期
- 【分类号】TQ464
- 【被引频次】32
- 【下载频次】612