【摘要】 采用二次饱和D 最优试验设计方法 ,在 3 0L生物反应器中进行了蛹虫草液态深层发酵。当初始温度为 2 2℃ ,pH 6 3～ 6 5 ,搅拌速度 1 80r/min ,通风量 1 2 1m3/h ,发酵 72h和 96h ,蛹虫草菌丝体 (干 )产率分别为 3 8 6g/L和 42 3g/L。用HPLC法检测腺苷和蛹虫草菌素的含量 ,72h时分别为湿菌丝体中 0 3 5 2 μg/g和 0 1 3 4μg/g ,发酵液中 0 1 79μg/mL和 0 1 0 2 μg/mL ;用比色法在41 2nm处检测虫草酸的含量 ,湿菌丝体中为 2 3 40 6mg/g,发酵液中为 6 61mg/mL。更多还原

【Abstract】 Liquid fermentation of theCordyceps militaris in a 30 liter bioreactor was studied using the design of quadratic saturation and D-optimal experiment. When the initial temperature of the fermentation was 22℃, pH was 6.3 to 6.5, the Cordyceps militaris mycelium reached 38.6 g/L after 72 hours and 42.3 g/L after 96 hours. The adenosine was 0.325μg/g and the cordycepin was 0 134μg/g in the wet mycelium of 0.179μg/mL and 0.102μg/mL in the ferment solution respectively after 72 hours. The cordycepic acid content was 23.406mg/g in the wet mycelium and 6.61mg/mL in the ferment solution at 72 hours.更多还原