【摘要】 以邻菲咯啉为配位剂,将毛细管电泳与鲁米诺-过氧化氢化学发光体系结合,成功的分离并检测了Fe(II)和Fe(III).在该体系中引入邻菲罗啉有以下优点:(1)有效的防止了Fe(II)被空气氧化的可能性.(2)提高了Fe(II)检测灵敏度.(3)提高了Fe(II)和Fe(III)的分离效率.在pH4.3的醋酸-醋酸钠缓冲溶液中,不到8min的时间即可实现Fe(II)和Fe(III)的分离.此法对Fe(II)检测的线性范围是1nmol/L～200nmol/L,对Fe(III)的线性范围是10nmol/L～330nmol/L,它们的检出限分别是Fe(II)68amol(3.4×10-9mol/L)和Fe(III)460amol(2.3×10-8mol/L).更多还原

【Abstract】 A new method for the simultaneous determination of iron(II) and iron(III) using the chelating reagent 1,10-phenanthroline is firstly reported. Capillary electrophoresis (CE) with chemiluminescence (CL) detection was used in the separation of iron(II) and iron(III) complexes which catalyze the CL reaction of luminol with hydrogen peroxide (H2O2). It was found that 1,10-phenanthroline could improve the separation efficiency of iron(II) and iron(III) and markedly enhance the chemilumiescence signal of iron(II), and meanwhile avoid that iron(II) is rapidly oxidized by oxygen. The metal ions were separated in less than 8 min with sodium acetate buffer (pH=4.3). The method was linear for iron(II) in the range 1nmol/L to 200nmol/L and for iron(III) from 10nmol/L to 330nmol/L. The procedure has detection limits of 68 amol (3.4×10-9 mol/L) and 460 amol (2.3×10-8 mol/L) for iron(II) and iron(III), respectively.更多还原