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圈养大熊猫粘液水解氨基酸含量的初步研究
Preliminary Study on Amino Acids of Mucus Excreted from Giant Panda
【摘要】 收集了成都大熊猫繁育研究基地的 8只圈养大熊猫的 12个粘液样品并测定了其中 17种水解氨基酸(AA)的含量。结果如下 :粘液干物质中Glu含量最高 ,为 9 2 4 %± 0 4 7% ,其次为Leu、Asn、Thr、Ser、Pro、Val、Lys、Ala、Tyr、Arg、Gly、Ile、Phe、Cys、His,Met含量 (1 2 5 %± 0 18% )最低 ,不同样品中同一种AA的含量不同。样品中 9种非必需氨基酸 (NEAA)的总平均含量为 4 1 1%± 2 2 % ,8种必需氨基酸(EAA)的总平均含量为 2 9 8%± 1 8% ,NEAA/EAA的平均值为 1 38± 0 0 4 ,不同粘液样品的这些数值相近。不同样品中同一种EAA与该样品中Lys含量的比值存在不同程度的差异 ,样品中 (Tyr+Phe) /Lys值最高 ,为1 79± 0 31;其次为Leu/Lys和Thr/Lys;而Met/Lys值最低 ,为 0 31± 0 0 5。各种氨基酸相对于总氨基酸含量的高低顺序与各AA绝对含量高低顺序完全相同 ,Glu含量最高为 13 0 %± 0 30 % ,Met含量为 1 76 %±0 2 5 %。结果表明 ,大熊猫粘液样品各种AA特别是EAA含量丰富 ,不同样品含量存在不同程度的差异 ;认为对排粘频繁和每次排粘量大的大熊猫 ,必须补充适量的优质蛋白以弥补由粘液排泄而造成的损失 ,以促进其体况的尽快恢复。
【Abstract】 Twelve mucus samples from 8 giant pandas were collected and contents of 17 kinds of hydrolysis amino acids (AA) were tested. The results were as follows: the content of Glu was 9.24%±0.47%,which ranked first of 17 kinds of AA, and then Leu,Asn,Thr,Ser,Pro,Val,Lys,Ala,Tyr,Arg,Gly,Ile,Phe,Cys and His;that of Met was 1.25%±0.18%,which was the lowest;the content of the same AA in distinct samples was different;the average content of all 9 kinds of non-essential amino acids (NEAA) was 41.1%±2.2% and that of 8 essential amino acids (EAA) 29.8%±1.8%;the ratios of NEAA/EAA were 1.32~1.45,with an average value of 1.38±0.04;the values of different samples are similar;the average ratio of (Tyr+Phe)/Lys was 1.79±0.31,which was the highest of all of the EAA,and then was Leu/Lys and Thr/Lys;the ratio of Met/Lys (0.31±0.05)was the lowest;the ratio of the same EAA of different samples was distinct;the sequences of ratios of every AA to total of AA were the same as the content of each AA;the content of Glu(13.0%±0.30% )was the highest while that of Met (1.76%±0.25%) was the lowest. The results showed that the contents of each AA especially EAA of mucus were plentiful. It has been suggested by the authors that good quality protein should be supplied to those giant pandas that often excreted mucus,and/or every time excreted much mucus.
- 【文献出处】 四川动物 ,Sichuan Journal of Zoology , 编辑部邮箱 ,2004年01期
- 【分类号】Q592
- 【被引频次】2
- 【下载频次】72