【摘要】 通常取日本血吸虫全长编码序列会面对以下三种情况 :其因可能为已知序列的基因 ,已知部分序列的基因或者不知序列的基因 ,本文针对以上常见的每一种情况。总结从cDNA文库、RNA和基因组中获取日本血吸虫全长编码序列的各种方法 ,为运用分子生物学方法研究日本血吸虫的学者提供参考更多还原

【Abstract】 Conventionally there are three conditions for obtaining complete coding genetic sequence of schistosoma japonicum,the gene may has been known,partial known or unknown. In line with each of the above mentioned conditions various methods in obtaining complete coding genetic sequence of schistosoma japonicum from cDNA library, RNA and genome are summarized in this paper intending to offer reference for those engaged in the research of schistosoma japonicun by using the way of molecular biology.更多还原