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氟虫腈对二化螟抗、感种群的亚致死效应
Sublethal effect of fipronil on resistant and susceptible rice stem borer (Chilo suppressalis Walker)
【摘要】 用氟虫腈亚致死剂量分别处理浙江苍南县抗性二化螟和安徽太湖县敏感性二化螟。结果表明 ,处理后两种群的存活率、产卵量、孵化率都明显降低 ,幼虫期延长 ;氟虫腈对抗性种群存活率的持续影响作用大于对敏感种群的。此外 ,亚致死剂量处理后 ,敏感种群的酯酶 (CarE)活性比对照明显升高 ,敏感、抗性种群的Km 值均增大 ,表明酯酶对底物的亲和力都有所降低。
【Abstract】 The rice stem borer,Chilo suppressalis,from both resistant(Cangnan,Zhejiang)and susceptible(Taihu,Anhui)populations was treated with the respective sublethal dose(LD 10 and LD 25 )of fipronil to see if there were sublethal effects.It was found that the sublethal dose obviously depressed the survivorship,fecundity and fertility of C.suppressalis,and prolonged the larval duration.The sustained effect of fipronil on survivorship of the resistant population was stronger than that of the susceptible one.In addition,specific activity of CarE in the susceptible population increased significantly after the treatment with sublethal dose of fipronil.The K m value of CarE increased in both resistant and susceptible populations when treated with the respective sublethal dose,indicating that the affinity of CarE to α-naphthyl acetate decreased.
【Key words】 fipronil; Chilo suppressalis Walker; sublethal dose; development; CarE;
- 【文献出处】 南京农业大学学报 ,Journal of Nanjing Agricultural University , 编辑部邮箱 ,2004年02期
- 【分类号】S481
- 【被引频次】50
- 【下载频次】332