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5S rDNA间隔序列分析和RAPD技术用于鉴定体细胞杂种
Identification of somatic hybrids by the methods of 5S rDNA spacer sequence and RAPD analysis
【摘要】 主要对狭叶柴胡 (Bupleurumscoronerifolium)与川西獐芽菜 (SweritamusstiiFranch)的科间体细胞杂种愈伤组织、小麦 (Triticumaestivum)与燕麦 (Avenasativa)的族间体细胞杂种愈伤组织及再生植株进行 5SrDNA间隔序列及RAPD分析鉴定 ,确证了它们为体细胞杂种
【Abstract】 The present paper described the 5S rDNA spacer sequence and RAPD analysis in the identification of hybrid nature of the calli line from interfamilial somatic hybridization between Bupleurum scoronerifolium and Swerita musstii Franch, the calli line from intertribal somatic hybridization between Avena sativa and Triticum aestivum L. and its regenerated plants. We confirmed that they were somatic hybrids.
【基金】 山东省自然科学基金重点项目,Z2 0 0 1D0 1
- 【文献出处】 植物研究 ,Bulletin of Botanical Research , 编辑部邮箱 ,2004年01期
- 【分类号】Q942
- 【被引频次】11
- 【下载频次】183