【摘要】 目的　检测鼻NK/T细胞淋巴瘤中的穿孔素、颗粒酶B表达分布情况 ,为临床治疗和预后判断提供依据。方法　运用免疫组化S P法检测 11例鼻NK/T细胞淋巴瘤中穿孔素、颗粒酶B的表达和分布情况 ;同时与组织芯片中其他类型淋巴瘤进行比较研究 ,10例淋巴组织反应性增生作为对照。结果　 11例鼻NK/T细胞淋巴瘤中均见穿孔素和颗粒酶B过度表达。以 6 0例淋巴瘤制成组织芯片的 4 2例B细胞淋巴瘤中见 11例少许穿孔素和 14例少许颗粒酶B阳性表达细胞散在分布于瘤细胞间 ,10例外周T细胞淋巴瘤中 8例少许表达穿孔素和 9例少许表达颗粒酶B。 2例间变性大细胞淋巴瘤见少许穿孔素和颗粒酶B阳性表达细胞。 2例霍奇金淋巴瘤阴性。NK/T细胞淋巴瘤的穿孔素和颗粒酶B表达程度与T、B淋巴瘤比较 ,差异有显著性 (P <0 0 1)。结论　穿孔素和颗粒酶B是鉴定活化的细胞毒性细胞的免疫标记物 ,可作为NK/T细胞淋巴瘤的诊断性标记物 ;其在外周T细胞淋巴瘤和B细胞淋巴瘤中的表达 ,反映了机体存在抗肿瘤的免疫反应机制。更多还原

【Abstract】 Purpose To detect the expression and distribution of activated cytotoxic cells in types of lymphoma with tissue microarray, and to provide evidences for clinical treatment and evaluation of prognosis. Methods Immunohistochemical staining by S-P technique was used to detect the expression and distribution of perforin and granzyme B in the lymphoma tissue microarray, composed of 60 cases of lymphoma. 9 cases of NK/T-cell lymphoma routine sections were used for relative research, and 10 cases of reactive lymphoid hyperplasia were also used for comparison. Results In the tissue microarray, originated from the nodal and extra-nodal sites were 48 cases and 12 cases, respectively; consisting of 42 cases of B-cell lymphoma, 16 cases of T-cell lymphoma, 2 cases of Hodgkin’s disease. In 42 cases of B-cell lymphoma, few perforin and granzyme B positive cells scattered among tumor cells, in 11 cases and 14 cases, respectively. In 10 cases of peripheral T-cell lymphoma, perforin and granzyme B positive were 8 cases and 9 cases, respectively. In 11 cases of NK/T-cell lymphoma (2 cases in the tissue microarray, 9 cases routine sections), both perforin and granzyme B were strongly positive. Both the expression in B-cell lymphoma, T-cell lymphoma and NK/T-cell lymphoma was significantly different(P<0.01). Conclusions Perforin and granzyme B are immune markers for the identification of activated cytotoxic cells, which also can be used as diagnostic markers of NK/T-cell lymphoma. Their expression in B-cell lymphoma reflectes the anti-tumor immunologic reaction of host.更多还原