【摘要】 目的 探讨人骨髓间充质干细胞(BMSC)分离培养的细胞周期及细胞表面抗原特性。方法 应用离心法分离培养人BMSC;应用流式细胞学技术测定细胞周期及细胞表面抗原特征。结果 培养的BMSC呈多种形态。细胞周期分析显示G0+G1的比例为88.4％,S+G2+M的比例为11.6％。CD分子阳性细胞百分率结果显示CD29、CD44、HLA-ABC、CD45、CD80、CD34、CD38、HLA-DR分别为86.00％、71.52%、80.45％、71.45％、2.27％、2.83％、5.86％、1.88％。结论 BMSC具有独特的细胞遗传学和免疫学特征。更多还原

【Abstract】 Objective To investigate the cell cycle and surface antigentic characteristics of isolation and culture of human bone marrow mesenchymal stem cells. Methods Culture of human bone marrow mesenchymal stem cells was initiated from bone marrow mononuclear cells separated by centrifugation. The cell cycle and surface antigentic features were analyzed by flow cytometry technique. Results The cultured cells appeared in various kinds of shapes. The cell cycle analysis showed that the percentages of G0 + G1 and S + G2 + M phases were 88.4%, 11.6%, respectively. The surface antigentic analysis showed that the positive cells percentages for CD29, CD44, HLA - ABC,CD45, CD80,CD34,CD38,HLA- DR were 86.00% ,71.52% ,80.45% ,71.45% ,2.27% ,2.83% ,5.86%,1.88% % , respectively. Conclusion BMSC had unique cytogenetic and immunologic characteristics and provided a base for further investigation on purifying cells.更多还原