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双孢蘑菇部分cDNA文库的构建及筛选
CONSTRUCTION AND SCREENING OF PARTIAL CDNA LIBRARIES OF AGARICUS BISPORUS
【摘要】 构建了双孢蘑菇Agaricus bisporus菌株AA和A41的部分cDNA文库,其滴度分别为1.0?05 pfu/ml和6.0×104pfu/ml。用地高辛标记的丛生差异片段探针对文库进行筛选,分别获得5个和3个阳性克隆。
【Abstract】 The partial cDNA libraries of Agaricus bisporus AA and A41 were constructed with titer of 1.0×105pfu/ml and 6.0×104pfu/ml respectively. Five and three positive clones were separated respectively by screening the two libraries with Dig-labeled cluster special probe.
【关键词】 双孢蘑菇;
丛生变异;
阳性克隆;
二氢硫辛酰胺脱氢酶;
【Key words】 Agaricus bisporus; clustering variation; positive clone; DLDH;
【Key words】 Agaricus bisporus; clustering variation; positive clone; DLDH;
【基金】 福建省自然科学基金资助
- 【文献出处】 菌物学报 , 编辑部邮箱 ,2004年01期
- 【分类号】Q943
- 【被引频次】14
- 【下载频次】184