【摘要】 以鸡蛋枣的茎段为外植体,进行了芽的诱导和成苗的研究.结果表明:在MS+ZT1.75mg·L-1+KT2.0mg·L-1培养基,茎段可以分化不定芽.低浓度的NAA促进不定芽的形成,在MS+ZT1.75mg·L-1+KT2.0mg·L-1+NAA0.05mg·L-1中培养基芽的增殖系数达到4.2倍.在1 2MS+IBA0.8mg·L-1培养基中,试管苗的生根率达到95.0%.试验还就外植体的消毒和组培苗的移植进行了探索.更多还原

【Abstract】 The induction of adventitious buds from stem segments and the forming of plantlets in vitro were studied in Jidanzao jujube .The results showed that adventitious buds could be induced from stem segments on the culture medium of MS+ ZT 1.75 mg·L^-1+ KT 2.0 mg·L^-1.The addition of low concentrations of NAA to the medium made higher induction rates of adventitious buds and 4.2 times,the optimal proliferation number,was gotten on the medium of MS + ZT 1.75 mg·L^-1+ KT 2.0 mg·L^-1+NAA 0.05 mg·L^-1.When the shoots formed in vitro got length of 3～4 cm,they were used for the induction of adventitious roots.The optimal medium for this purpose was 1/2 MS+IBA 0.8 mg·L^-1 and the forming rate of plantlets was over 95.0%.In addition,the sterilization of explants and the transplant of plantlets were also studied.更多还原