【摘要】 研究枯草杆菌蛋白酶催化环糊精与癸二酸二乙烯酯在 DMF有机溶剂中的区域选择性反应 ,产物结构经IR、1 H NMR、1 3C NMR、MS和 DSC分析证实为单 - (2 - O-乙烯癸二酰 ) - β-环糊精 ;同时考察了三种不同的酶对反应的催化作用 .结果表明枯草杆菌蛋白酶和 Candida cylindracea脂肪酶对 β-环糊精在 C- 2位仲羟基的酯交换反应显示很高的选择性 ,而 lipozyme固定化酶在此体系中没有催化活性 .更多还原

【Abstract】 Transesterification of cyclomaltoheptaose （β-CD） with divinylsebacate was catalyzed by the alkaline protease from Bacillus subtilis in anhydrous DMF for 5 days forming the corresponding vinyl-β-CD derivative. The product was characterized with ESI-MS, （）¹H NMR, （）¹³C NMR, IR and DSC. The results indicated that the product to be monosubstituted ester, with monoacylation occurring at the C-2 secondary hydroxyl groups of β-CD. The selectivity of CCL and lipozyme were also studied. The result indicated that CCL shows the same selectivity as alkaline protease from Bacillus subtilis, but lipozyme has no catalytic activity under the same reaction condition.更多还原