【摘要】 目的　研究三氧化二砷 (As2 O3)对人肺腺癌Anip973细胞内游离 [Ca2 + ]i的影响。方法　培养人肺腺癌Anip973细胞 ,用激光扫描共聚焦显微镜 (LSCM)观察As2 O3对人肺腺癌Anip973细胞内游离 [Ca2 + ]i的影响 ;用生长曲线法测药物对肿瘤细胞增殖的影响。结果　①LSCM显示As2 O3作用后的人肺腺癌细胞Anip973细胞内 [Ca2 + ]i显著升高 (P <0 .0 0 1 )。②As2 O3对人肺腺癌细胞株Anip973细胞内 [Ca2 + ]i升高作用具有剂量依赖性。③As2 O3能明显抑制人肺腺癌Anip973细胞的增殖。结论　As2 O3可能是通过升高人肺腺癌细胞内 [Ca2 + ]i来启动细胞凋亡机制 ,诱导肺癌细胞凋亡 ,从而抑制其增殖更多还原

【Abstract】 Objective To explore the effect of arsenic trioxide (As 2O 3) on intracellular free calcium concentration.Methods A single cell [Ca 2+ ]i was measured by laser scanning confocal microscope using Ca 2+ sensitive dye Fluo 3 /AM as a new calcium fluorescent probe; The antiproliferation activities of As 2O 3 on Anip973 cells were evaluated by means of continuous growth curve.Results As 2O 3 could obviously increase the concentration of intracellular free calcium in dose dependent manner.Conclusion The antitumor mechanism of arsenic trioxide may be that it can induce Anip973 cell apoptosis through increasing [Ca 2+ ]i level; As 2O 3 can inhibit the proliferation of Anip973 cells significantly.更多还原