【摘要】 目的　克隆抗原处理相关转运体亚基TAP2基因片段并构建真核表达载体。方法　从EB病毒刺激的人B淋巴母细胞系 (B LCL)中提取总RNA ,用RT PCR从中扩增出TAP2cDNA。将TAP2cDNA插入表达载体pcDNA3.1 V5 His TOPO中 ,构建重组表达载体pcDNA3.1 V5 His TOPO TAP2 ,并进行测序分析。结果　经RT PCR和测序鉴定 ,成功地克隆了人TAP2cDNA。结论　获得了人TAP2cDNA并构建了重组真核表达载体 ,对TAP2基因产物在体外表达、进一步研究TAP2分子在肿瘤基因治疗方面的作用具有重要的意义。更多还原

【Abstract】 Objective To clone the transporter associated with antigen processing 2 (TAP2) gene and construct its eukaryotic expression vector.Methods Human TAP2 cDNA was amplified by RT PCR from Epstein Barr virus (EBV) treated human B lymphoblastoid cell line (B LCL).The TAP2 cDNA was inserted into the expression plasmid pcDNA3.1/V5 His TOPO to construct the recombinant expression vector pcDNA3.1/V5 His TOPO TAP2.Results RT PCR and DNA sequencing showed that human TAP2 cDNA was cloned successfully.Conclusion Human TAP2 cDNA is obtained and the recombinant eukaryotic expression vector is constructed,which provide the possibility for further expression of TAP2 in vitro ,and lay the foundation for further studying the function of TAP2 protein in cancer gene therapy.更多还原