【摘要】 以 ODS-2 Spherisorb C18 反相 HPLC 分析柱,流动相 A (体积比为 80∶20 的甲醇,pH = 7.2 的 0.5 mol/L 乙酸氨水溶液,0.01 % BHT W∶V)、流动相 B (87.5∶12.5 的乙腈:水,0.01 % BHT W∶V)和流动相 C (乙酸乙酯),梯度洗脱,流速为 1 mL/min,紫外检测波长为 436 nm,HPLC 方法分析辽东湾表层海水中的叶绿素 a 含量。该方法灵敏度高,检测限为 10 ng/L。对 HPLC、荧光法和分光光度法的叶绿素 a 分析结果进行了比较。更多还原

【Abstract】 A HPLC method has been used for determination of chlorophyll-a in the surface sea water of the Liaodong Bay. ODS-2 Spherisorb C18 reversed phase column is used. UV detection wavelenth is 436 nm. The HPLC solvents are methanol, 0.5 mol/L ammonium acetate aaq., acetonitril, water and ethyl acetate. They are all of HPLC-grade. Limit of detection is 10 ng/L. The correlations of three methods (spectrometer, fluorescence, HPLC) are compared.更多还原