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布洛芬乙酯的微生物拆分

Microorganism for enantioselective separation of ibuprofen ethyl-ester

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【Author】 TAN Zhongfang 1) , WANG Yanping 1) , WU Jian 1) , SHI Xianming 2) , LIU Hongming 3) , QIN Guangyong 1) , HUO Yuping 1) 1)Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou 450052 2)Department of Food Science & Technology, Huazhong Agricultural University, Wuhan 430070 3)Department of Chemistry, Zhengzhou University, Zhengzhou 450052

【机构】郑州大学离子束生物工程省重点实验室；华中农业大学食品科技系；郑州大学化学系；郑州大学离子束生物工程省重点实验室郑州450052；郑州450052；武汉430070；郑州450052；

【摘要】目的 :从土壤中筛选出能立体选择性水解布洛芬乙酯 ,并且水解产物为S -布洛芬的菌株。方法 :土样通过吐温 2 0培养基初筛到酯酶活力较高的菌株 ,并通过复筛测定菌株水解布洛芬乙酯的产物旋光度 ,从而得到活性菌株。结果与结论 :用筛选到的菌株Candidasp .的培养物催化水解布洛芬乙酯所得产品的外观、熔点、旋光度和核磁共振色谱等各项检测指标与Sigma公司的标准品S -布洛芬基本一致 ,表明该菌株具有生产S -布洛芬的潜力。更多还原

【Abstract】 Aim: To screen strains of high preference for enantioselective hydrolysis of ibuprofen ethyl-ester to the corresponding S-ibuprofen from soil samples. Methods: The strain of high preference easterase was screened through 20 culture mediums and the rotation of hydrolysis production of ibuprofen ethyl-ester was detected. Results: The characteristics of purified hydrolyzer by the strain Candida sp., such as appearance, melting point, the rotation, and the nuclear magnetic resonance spectrum, approached to those of the standard S-ibuprofen from Sigma company. Conclusion: Candida sp. has potential for the commercial production of S-ibuprofen.更多还原

【关键词】 S-布洛芬；酯酶；立体选择性水解；
【Key words】 S-ibuprofen； esterase； enantioselectivity；

【文献出处】郑州大学学报(医学版) ,Journal of Zhengzhou University(Science Medical) , 编辑部邮箱 ,2004年01期
【分类号】TQ463
【被引频次】6
【下载频次】171

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