【摘要】 目的探讨砷化物对人类正常肝细胞金属硫蛋白MetallothioneinMT-3cDNA基因表达的影响。方法利用SMART方法克隆cDNA基因,生物信息学分析克隆基因的同源性、染色体定位、基因结构和对该基因编码蛋白质进行分析并对该蛋白质进行跨膜信息分析,基因芯片方法检测染砷L-02细胞MT-3基因的表达。结果成功克隆MT-3cDNA基因,经生物信息学分析可知,该基因定位在人16号染色体16q13区段,跨膜信息分析发现MT-3基因编码蛋白质可以穿出生物膜,基因芯片结果证实染砷的人正常肝L-02细胞在染砷早期MT-3基因表达增高。结论该研究发现人类MT-3基因是砷化物作用的一个相关基因,早期参与了砷代谢解毒。更多还原

【Abstract】 Objective To study the effects of arsenic compounds on metallothionein-3 gene expression in human normal hepatic cells. Methods cDNAs were cloned by SMART method. Bioinformatics was utilized to analyze the homologue, chromosomal localization, structure and encoding protein of the cloned gene and the trans-membrane information of the encoding protein. Metallothionein-3 gene expression level in L-02 cell line treated by arsenite was determined by cDNA microarrays. Results Metallothionein-3 cDNA gene was cloned and located in the chromosome 16q13. The results of bioinformatics analysis showed that the protein encoded by metallothionein-3 gene could cross the biologic membrane. Metallothionein-3 gene expression up-regulation in human normal hepatic L-02 cell line was found by cDNA microarrays in the early stage after the cells being exposed to arsenite. Conclusion The results of this study showed that the human metallothionein-3 gene was arsenic related gene and this gene might play a vital role in the detoxification metabolism of arsenic compounds at early stage.更多还原