【摘要】 以转 hTNF-a 基因聚球藻 7002为对象,在摇瓶培养下对其生长条件进行了初步研究. 结果表明,当光照强度为100 mol/(m2s)时,藻细胞生长速率最大;35oC为其最佳培养温度;氯化钠浓度为12~24 g/L时生长良好,最适浓度为24 g/L;转hTNF-基因的聚球藻7002能利用铵盐和硝酸盐为氮源,其中硝酸盐对其生长最有利,硝酸钠最适浓度为1 g/L;加入有机碳源可以显著促进藻细胞生长,其中5 g/L的蔗糖对其促进作用最明显.更多还原

【Abstract】 Light, temperature, NaCl concentration, nitrogen source, organic carbon source are important factors affecting the growth of transgenic Synechococcus sp. PCC7002 with hTNF- gene. The growth of transgenic Synechococcus sp. PCC7002 was saturated at the level of 100 mol/(m2s) light intensity. The optimal temperature for cultivation was 35oC. The range of NaCl concentration suitable to cell growth was from 12 g/L to 24 g/L, and the optimal was 24 g/L. Transgenic Synechococcus sp. PCC7002 could use ammonium or nitrate for its nutritional requirement, but nitrate was the preferred nitrogen source and the optimal concentration was 1 g/L NaNO3. Several organic carbon compounds such as sucrose, glucose, glucosamine were tested. It was shown that organic carbon source promoted the cell growth apparently and 5 g/L sucrose was proved to be the most suitable.更多还原