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分光光度法测定发酵液中果糖的含量
Determination of Fructose in Broth by Spectrophotometry
【摘要】 针对微生物转化发酵液体系的特征 ,用分光光度法测定其中果糖的含量。校准曲线回归方程为 y=2 6 .2 3x+0 .0 0 7,相关系数为 0 .9991 ;1 0倍量葡萄糖和 1 0倍量甘露醇的存在对果糖测定基本无干扰 ;发酵液中果糖测定的加标回收率为 99.1 %— 99.8%。
【Abstract】 The amounts of fructose in biotransformation broth was determined by spectrophotometry. The calibration curve is y=26.23x +0.007,with r =0.9991.The existence of 10 times glucose or 10 times mannitol does not interfere the determination of fructose.The recovery of fructose in the broth is 99.1%—99.8%.
- 【文献出处】 光谱实验室 ,Chinese Journal of Spectroscopy Laboratory , 编辑部邮箱 ,2004年05期
- 【分类号】O657.32
- 【被引频次】7
- 【下载频次】485