节点文献
牛初乳中IGF和TGF的分离与纯化
The isolation and purification of IGF and TGF from bovine colostrums
【摘要】 采用葡聚糖凝胶G - 75层析色谱、SPSepharoseFF离子交换色谱和制备型疏水作用色谱Oc tylSepharoseFF研究了IGF -Ⅰ ,IGF -Ⅱ和TGF - β的分离纯化 ,并且用高效液相色谱法对IGF -Ⅰ ,IGF -Ⅱ和TGF - β的含量进行了测定 .
【Abstract】 The study about the isolation and purification of IGF-Ⅰ,IGF-Ⅱand TGF-β from bovine colostrums were carried out by poly glucose gel chromatography,DEAE Sepharose F F Ion exchange chromatography,Octyl Sepharose F F and.Sephacryl S-100HR Gel filtration chromatography.The content of IGF-Ⅰ,IGF-Ⅱ and TGF-β were determinated by affinity chromatography and HPLC.
【关键词】 牛初乳;
IGF—Ⅰ;
IGF—Ⅱ;
TGF-β;
分离纯化;
【Key words】 colostrums; IGF—Ⅰ; IGF—Ⅱ; TGF-β; isolation and purification;
【Key words】 colostrums; IGF—Ⅰ; IGF—Ⅱ; TGF-β; isolation and purification;
【基金】 科技部国际合作计划项目 ( 2 0 0 1DFBA0 0 0 7-3 )
- 【文献出处】 高师理科学刊 ,Journal of Science of Teachers College and University , 编辑部邮箱 ,2004年01期
- 【分类号】TS252.1
- 【被引频次】3
- 【下载频次】142