【摘要】 目的:观察银杏叶提取物(EGb761)对过氧化氢(H2O2)诱导的晶状体上皮细胞增殖细胞核抗原(PCNA)表达的影响。方法:将离体培养的SD大鼠的晶状体上皮细胞分成3组:实验组用H2O2+EGb761处理,对照组用H2O2处理,空白对照组未做任何处理。用免疫组化的方法检测上皮细胞PCNA表达,分析实验组、对照组和空白对照组的表达情况。结果:PCNA阳性细胞率:实验组由于EGb761的抑制作用,在3h为15.3±4.0(%),对照组在H2O2诱导下,3h增加至34.2±5.9(%),空白对照组仅为9.9±2.3(%)。结论:H2O2作用早期可引起晶状体上皮细胞增殖,EGb761在H2O2作用早期能减少氧化应激引起的细胞增殖。更多还原

【Abstract】 AIM: To investigate the effect of ginkgo biloba extract (EGb 761) on the expr ession of PCNA of lens epithelial cells treated with H2O2.· METHODS: Cultured SD rat lens epithelial cells were divided into 3 groups: th e Study Group treated with H2O2 and EGb 761, the control group with H2O2 and the Blank Group without treatment. The expression of PCNA was studied by immunohist ochemistry, and then was analysed respectively.· RESULTS: The ratios of the positive-PCNA lens epithelial cells in Study Group , Control Group and Blank Control Group at treatment of 3h were 15.3±4.0(%), 34 .2±5.9(%) and 9.9±2.3(%), respectively. The difference is significant between the Study and Control Group.· CCONCLUSION: H2O2 can cause proliferation at early stage, and EGb 761 can red uce cell proliferation which caused by oxidative stress during the early period of H2O2 treatment.·更多还原