【摘要】 目的　探讨环氧合酶 2与子宫内膜癌发生、发展中的关系。方法　研究对象分为 5组 ,增生期组 2 5例 ,分泌期组 2 5例 ,内膜炎组 2 5例 ,非典型性增生组 2 3例 ,子宫内膜癌组 34例 ,应用免疫组化和定量RT PCR方法 ,检测其中Cox 2蛋白和mRNA水平表达。结果　 6 7%的子宫内膜癌表达Cox 2 ,在转录和蛋白水平子宫内膜癌组的Cox 2表达强度明显高于其它四组 (免疫组化评分分别为 :增生期组 5 4 6± 0 12 3,分泌期组 3 2 0± 0 176 ,内膜炎组 4 78± 0 12 ,非典型性增生组 6 10±0 2 5 ,子宫内膜癌组 8 70± 0 93,相应mRNA含量依次为 92 8± 8 2 2fpg/ μg ,6 4 9± 11 0 8fpg/ μg ,79 4± 5 83fpg/ μg ,2 99 3± 10 6 8fpg/ μg ,4 93 0± 2 9 5 8fpg/ μg) ,差异有显著性 (P <0 0 5 ) ,内膜癌中 ,高分化细胞Cox 2表达高于低分化细胞 ,差异有显著性 (P <0 0 5 )。非典型性增生组Cox 2表达显著高于正常内膜和内膜炎组 ,增殖期组Cox 2表达高于分泌期组 ,差异有显著性 (P <0 0 5 )。结论　Cox 2可能在子宫内膜癌发生发展中起重要作用 ,可为子宫内膜癌的化学预防和化疗的辅助治疗提供新靶点更多还原

【Abstract】 Objective To investigate the role of the expression of cyclooxyenas e-2 in the carcinogensis and development of endometrial carcinomaMethod Immunostaining, westernblotting and quantitve reverse trans cription-polymerase chain reaction (RT-PCR) assay were utilized to measure the levels of protein and mRNA expression of cyclooxyenase-2 in five groups: 25 cases in the proliferative phase, 25 cases in the secretory phase, 25 cases wi th endometritis, 23 cases with atypical proliferation and 34 cases with endomet rial carcinoma Result Cox-2 expression of both RNA and protein in patients with endometrial carcinoma was significantly higher than in patients in the prolifer ative and secretary phase or with endometritis and atypical proliferation. The immunostaining score was 546±012 vs 320±018,478±012,610±0 25 and 870±093,the average absorbent value was 075±023vs 041±045, 056±031, 110±056 and 146±041; and the concent ration of mRNA was 928±822 vs 649±1108,794±583,2993±1068 a nd 4930±2958 fpg/ugrespectively. Successively the expression of Cox -2 in the atypical proliferation group was higher than that in the normal endom etrium and endometritis groups. The expression was significantly higher in the p roliferative than in the secretory phase group (P<005).Conclusion Cox-2 may play an important role in the development of endometrial carcinoma. 〔更多还原