【摘要】 目的了解血清可溶性核小体与系统性红斑狼疮(SLE)活动性及抗核小体抗体(AnuA)的关系;探讨核小体与SLE临床表现及免疫学指标的关系。方法应用双抗夹心酶联免疫吸附试验(ELISA)检测33例SLE患者、31例疾病对照组(包括原发性干燥综合征、多发性肌炎、系统性硬化症、未分化结缔组织病、类风湿关节炎)和30名正常对照组血清中的核小体浓度,并记录SLE患者的各种临床表现及实验室指标,分析其与核小体的关系。结果SLE患者的血清中核小体的浓度明显低于正常对照组及疾病对照组(P<0.05);疾病对照组与正常对照组比较差异无显著性(P>0.05)。SLE患者的血清核小体浓度与AnuA的高低呈明显的负相关(r=-0.410,P=0.018);核小体减低组的SLE患者发热、皮疹、补体C4降低、血沉(ESR)增快、IgA增高、尿蛋白阳性的发生率明显高于核小体增高组,差异有显著性(P<0.05);核小体减低组中活动性狼疮的例数高于核小体增高组,差异有显著性(P<0.05)。结论SLE患者血清核小体浓度可减低或增高,血清核小体浓度与AnuA高低呈明显的负相关;血清核小体的减低与SLE皮肤损害、肾损害的发生明显相关;核小体的减低与SLE疾病活动性相关。更多还原

【Abstract】 Objective To analyze the serum nucleosome and antinucleosome antibody (AnuA) in systemic lupus erythematosus (SLE) and their role in the pathogenesis of disease. Methods Sandwich enzyme-linked immunosorbent assay technique was used to detect the level of nucleosome in serum of 33 SLE patients, 31 other rheumatic diseases (including primary Sj?觟gren′s syndrome, ploymyositis, dermatomyositis and systemic sclerosis) and 30 healthy controls. Results The level of sera nucleosome of SLE was much lower than that of patients with other rheumatic diseases and healthy controls (P<0.05). A reverse correlation was found between serum level of the nucleosome and serum anti-nucleosome antibody (r=-0.410, P=0.018). Conclusion Serum level of nucleosome is decreased or increased in SLE. An inverse correlation has been found between the serum level of nucleosome and serum anti-nucleosome antibody activity. The level of serum nucleosomes is closely related with disease activity. Nuleosome is an autoantigen involved in the pathogenesis of disease, especially in lupus nephritis.更多还原