【摘要】 从锥虫可变表面糖蛋白 ( VSG)中发现反式剪接以来 ,已经发现很多低等真核生物体内都存在这种剪接形式。这种广泛存在于低等真核生物中的反式剪接 ,对于生物蛋白多样性、阶段性表达有着重要的生物学意义。反式剪接中的反式剪接引导序列 ( spliced leader,SLRNA)为一段保守序列 ,SL RNA为寄生虫的功能基因的筛选、c DNA文库的构建提供了表达基因标签 ( EST) ,同时反式剪接为转基因治疗提供了有力的手段。反式剪接的发现和广泛研究 ,对于深入了解低等真核生物的核酸分子生物学及其起源、进化、系统发生、寄生虫病的预防研究等都有着重要意义。文章对反式剪接在低等真核生物体中的研究现状、SL RNA基本结构、生物学特点及其在现代分子生物学领域的应用进行了概述更多还原

【Abstract】 RNA trans-splicing in many primary eukaryotes has been found,and testified, since it was discovered from splicing of RNA of variable surface glycoprotein (VSG) in trypanosome. This trans-splice is very important for protein diversity and phasic expression in this organism. On the other hand, SL RNA, which is a constant sequence as a spliced leader, is very useful as EST for selection the functional gene or construction of cDNA library, and trans-splicing will contribute to gene therapy. Identification and wide researches of trans-splicing will play an important role in molecular biology of those primary eukaryotes, their origin, phylogenesis, the researches of prevention of many parasitoses and so on. This paper reviewed the current researches and reports on SL RNA (splicing leader), including its chemical structure, biological charactistics,and the applications in biology.更多还原