【摘要】 目的:探讨凋亡素-2配体(Apo2L)联合三氧化二砷(As2O3)诱导急性髓系白血病(AML)细胞凋亡的作用。方法:分离25例AML患者的骨髓单个核细胞,每例分为4组进行观察:空白对照组、Apo2L组、As2O3组、Apo2L+ As2O3组,共同培养12 h、24 h和48 h。通过MTT比色法测定细胞生长抑制率及流式细胞仪检测细胞凋亡。结果:Apo2L和As2O3可协同降低AML细胞活力,诱导细胞凋亡,其作用随培养时间延长及药物浓度增加而增强。结论:Apo2L与三氧化二砷具有协同作用,能高效杀灭白血病细胞。Apo2L是一种有望应用于临床的新型生物制剂。更多还原

【Abstract】 Objective: This study aims to investigate the apoptosis-inducing effect of Apo2L and arsenic trioxide (As2O3) on acute myeloid leukemic(AML) cells. Methods: 25 cases of BMMNC of AML cells were isolated. Each case was divided into 4 groups, which were control group, Apo2L group, As2O3 group and Apo2L+ As2O3 group. MTT assays were used to evaluate the cytotoxic effect at 12, 24 and 48 hours after the drugs were added. Flow cytometry were used to evaluate apoptosis. Results: Apo2L and As2O3 can synergistically inactivate AML cells, and induce apoptosis. This kind of effect presented a dose- and time-dependent relationship. Conclusion: Apo2L can present a synergistic effect with As2O3. The cytotoxic effect was mostly induced by apoptosis. Apo2L has a promising prospect in clinical use.更多还原