【摘要】 目的 :观察复方鱼油对大鼠糖尿病内耳病变的预防作用及其机制。方法 :5 0只雄性 Wistar大鼠随机分成 3组 ,A组为正常对照组共 16只 ,B组为糖尿病对照组 18只 ,C组为复方鱼油治疗组 16只。B、C两组静脉内注射链脲佐菌素 (STZ) 5 5 m g· kg- 1 体重 ,3周后 C组动物每日灌饲复方鱼油持续 9周。测试血中总胆固醇(TC)、甘油三酯 (TG)、低密度脂蛋白 -胆固醇 (L DL- C)和极低密度脂蛋白 -胆固醇 (VL DL- C)水平 ,记录动物听觉脑干诱发电位。结果 :C组动物 TC、TG、L DL 和 VL DL 较 B组明显降低 (P<0 .0 5 ) ;C组动物听觉脑干诱发电位 波潜伏期较 B组缩短 (P<0 .0 5 ) , 波阈值降低 (P<0 .0 5 ) ;透射电镜下 B组动物内耳毛细胞内大量脂滴聚集 ,线粒体、高尔基体空泡样变性 ,微血管壁内皮细胞肥大增生 ,基底膜增厚 ,C组则无明显变化。结论 :复方鱼油能明显降低血脂 ,并能有效减轻糖尿病引起的内耳病变。更多还原

【Abstract】 Objective To observe the preventive effect of combination fish oil on pathological lesions in inner ear of diabetic rats and its mechanism. Methods Fifty male Wistar rats were randomly divided into three groups randomly :group A (normal control, n=16), group B (diabetic control, n=18), and group C (treatment group,n=16). Rats in group B and group C were injected intravenously with streptozocin(STZ) of 55 mg·kg -1. Three weeks after injection,the rats in group C were continuously fed on combination fish oil everyday for 9 weeks. The levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C) and very low density lipoprotein-cholesterol (VLDL-C) in blood of rats were detected.The animal auditory brain stem evoked potentials were recorded. Results The levels of TC,TG,LDL, and VLDL in group C descented obviously compared with group B (P<0.05), auditory brain stem evoked potentials wave Ⅲ was obviously shortened in latent period compared with group B (P<0.05) and the threshold of wave Ⅲ decreased (P<0.05). Mass of lipid droplet in hairy cells in inner ear, vacuolar degeneration of mitochondrion and Golgi′s apparatus, hypertrophy and hyperplasia of endothelial cells in micrangium, and thick basement membrane were observed under transmission electron microscopy in group B. But these pathological lesions did not appear in the rats in group C. Conclusion Combination fish oil can prevent the lesions of inner ear in diabetic rats.更多还原