【摘要】 目的 :观察薯蓣皂苷元 (diosgenin,Dio)对人低分化胃腺癌细胞株 (MGC- 80 3)的细胞分裂和集落形成的影响 ,初步探讨其杀伤肿瘤细胞的机制。方法 :采用四唑蓝 (MTT)比色法、平板集落形成实验以及[3H]- Td R掺入实验。结果 :MTT法检测 Dio作用 2 4、 4 8、 72 h的半数抑制浓度 (IC50 )为 5 6 .2 90、 2 7.837、1 7.72 3mg· L- 1 ;平板集落形成实验显示 Dio半数集落形成抑制浓度为 5 .4 86 mg· L- 1 ;Dio在较低浓度 (3.75 0和 7.5 0 0 mg· L- 1 )下 ,可直接抑制 MGC- 80 3细胞 DNA的合成。结论 :Dio能够明显抑制 MGC- 80 3的细胞分裂和集落形成更多还原

【Abstract】 ObjectiveTo study the effect of diosgenin on the cell diversion and colony forming of human poorly differentiated gastric adenocarcinoma cell line MGC 803,and investigate the mechanism of cell death induced by diosgenin. Methods MTT assay,colony forming experiment and [ 3H] TdR incorporation into DNA were used. ResultsAfter incubated with diosgenin for 24, 48, and 72 hours, the IC 50 were 56 290, 27 837, and 17 723 mg·L -1 ,respectively, detected by MTT. The concentration which inhibited half colony forming was 5 486 mg·L -1 . It directly inhibited the synthesis of DNA of MGC 803 in lower concentrations (3 750 and 7 500 mg·L -1 ). ConclusionDiosgenin has an obvious inhibitory effect on the cell diversion and colony forming of MGC 803.更多还原