【摘要】 为探讨维生素C (VC)和维生素E (VE)联用对应激和非应激中华鳖幼鳖的生长、肝脏VC 和VE 以及血清皮质醇含量的影响 ,作者使用了 5组饵料 ,VC 和VE 的添加量依次为 0和 0mg/kg (对照组 )、 2 5 0和 5 0mg/kg (实验Ⅰ组 )、2 5 0 0和 5 0mg/kg (实验Ⅱ组 ) ;2 5 0和 2 5 0mg/kg (实验Ⅲ组 ) ;2 5 0 0和 2 5 0mg/kg(实验Ⅳ组 )。中华鳖幼鳖的生长、肝脏VC 和VE以及血清皮质醇分别通过特定生长率、高压液相色谱法和放免法来测定。结果实验Ⅰ -Ⅳ组中华鳖的特定生长率明显高于不加VC 和VE的对照组 ,但实验Ⅰ -Ⅳ组间没有明显不同。非应激中华鳖肝脏VC 和VE的含量随饵料中VC 和VE 含量的增加而明显升高 ,并且实验Ⅱ -Ⅳ组肝脏VC 和VE都明显高于对照组和实验Ⅰ组。酸应激后 ,对照组和实验组中华鳖肝脏VC 和VE都有下降的趋势 ,但无显著差异 ;应激后实验Ⅱ -Ⅳ组肝脏VC 和VE均明显高于对照组 ,实验Ⅳ组明显高于其它 4组。血清皮质醇的含量在实验Ⅰ -Ⅳ组间没有明显不同 ,实验Ⅰ -Ⅲ组与对照组相比虽有降低的趋势但没有变化 ,实验Ⅳ组则明显低于对照组。酸应激后 ,对照组血清皮质醇明显升高 ,其他 4组虽有升高的趋势 ,但没有明显变化。应激后实验Ⅰ -Ⅳ组血清皮质醇的含量均明显低于对照组 ,实验Ⅰ -Ⅳ组间没有明显更多还原

【Abstract】 We studied the effects of the combination of vitamin C and E on growth, liver vitamin C and E, and level of serum cortisol in stressed and non-stressed juvenile soft-shelled turtles. The turtles were fed combination of vitamin C and E at the dosages of 0 and 0 mg/kg diets(control group), 250 and 50 mg/kg diets(experiment group Ⅰ), 2 500 and 50 mg/kg diets(experiment group Ⅱ), 250 and 250 mg/kg diets(experiment group Ⅲ), 2 500 and 250 mg/kg diets(experiment group Ⅳ)diets, respectively for 4 weeks. The growth of juvenile soft-shelled turtle was estimated by specific growth rate, the level of liver vitamin C and E was analyzed by the method of high pressure liquid chromatogram(HPLC), and serum cortisol was determined by radioimmunoassay. The specific growth rate in the four groups of experiment Ⅰ-Ⅳ were higher than that of the control, whereas no significant difference was observed among experiment Ⅰ-Ⅳ groups. Levels of liver vitamin C and E in non-stressed turtles increased with increase of dietary vitamin C and E, and experiment Ⅱ-Ⅳ groups were significantly higher than those of the control group and experiment group Ⅰ. After acid stress, levels of liver vitamin C and E in all groups had a tendency to decrease, but there were no significant changes; levels of liver vitamin C and E in experiment Ⅱ-Ⅳ groups were higher than those of the control group, and experiment Ⅳ group was higher than the other four groups after acid stress. The level of serum cortisol showed no significant difference among experiment Ⅰ-Ⅳ groups, and though experiment Ⅰ-Ⅲ groups had a decrease tendency compared with control group, there was no significant change; whereas experiment Ⅳ groups was lower than that of control. After acid stress, serum cortisol in control group was significantly elevated, whereas the other four groups showed no significant change, although there was a tendency to increase. Serum cortisol levels in experiment Ⅰ-Ⅳ groups were lower than those of the control group after stress, whereas there was no significant difference among Ⅰ-Ⅳ groups. These results suggest that the absence of vitamin C and E depressed growth, and their combination had no interactive effect on the growth and serum cortisol levels, whereas they had an effect on levels of liver vitamin C and E in soft-shelled turtles.更多还原