【摘要】 用生物学、血清学及电镜技术鉴定了西瓜花叶病毒分离物 (XZ -1)的基本属性。研究结果表明 ,该分离物可侵染 6科 17种植物。其中 ,在葫芦科、茄科、菊科、苋科几种植物上表现为花叶 ;在苋色藜、蚕豆上表现为枯斑。桃蚜 (MyzusPersicae)可传毒 ,病毒汁液极易摩擦接种。该分离物汁液的致死温度为 5 5～ 60℃ ,稀释限点为 10 - 3～ 10 - 4,体外存活期约为 2～ 3d。分离物形态为典型球状 ,其提纯物紫外光吸收的最高峰在 2 60nm ,最低峰在 2 40nm。A2 80 /A2 6 0 =0 .69。该病毒核酸含量约为 11.3 %。ELISA测定该分离物与黄瓜花叶病毒的抗血清学反应为阳性 ,初步鉴定该分离物隶属于黄瓜花叶病毒 (CMV)更多还原

【Abstract】 In this paper, the basic properties of the isolation substance from XZ-1 were identified with the methods of biology, serums and electric microscope. The results showed that the isolation substance could infect 17 species in 6 families.Among them,Cucurbitaceae, Solanaceae,Composeitae and Amaranthaceae,displayed mosaic.At other plants such as Chenopodium anarenticolor and Vicia faba showed withered spots.On the other hand,Myzus persicae could spread poison to other plants.Thermal inactivation point(TIP) of the isolation substance liquids was 55～60?℃.Dgltution end point(DEP)was 10 -3～10 -4 and longevity in vitro(LIV) was 2～3?d.The shape of the virus was typical sphere,A 260/A 280= 0.69.RNA content was about 11.3%.The serum reaction between the isolation substance and CMV was positive by ELISA.It is primarily confirmed that the isolation substance belongs to CMV.更多还原