【摘要】 对一株里氏木霉突变株液体发酵产纤维素酶的培养条件进行了优化,确定培养基的最适碳源和氮源种类及添加量(g/L)分别为:小麦秸秆15,小麦麸皮5,尿素10～15,(NH4)2SO412,NH4NO35;最适培养条件为:接种种龄72h,接种孢子悬液浓度2×107个/mL,培养温度30～32℃,pH值5 0,培养时间144h,摇瓶转速180r/min,装液量75mL/250mL三角瓶.在培养里氏木霉的培养基中同时接种黑曲霉(二者孢子数比为60∶1)进行混合培养,β 葡萄糖苷酶的产量是对照试验的2 9倍,明显改善了纤维素酶系的比例,提高了它们之间的协同作用效果,在最适培养条件下,粗酶液的β 葡萄糖苷糖活和FPA酶活分别达到127 6U/mL和84 8U/mL.更多还原

【Abstract】 The proper carbon and nitrogen source in liquid medium and optimal cultivating conditions for cellulase synthesis by a Trichoderma reesei strain were studied. The optimal carbon and nitrogen source (g/L) were: wheat straw 15, bran 5, urea 10-15,(NH4)2SO4 12 and NH4NO3 5. The optimal cultivating conditions were: inoculum age 72h, inoculum size 2×107 spore/mL, cultivating temperature 30-32℃ , pH 5.0, cultivating period 144h, inoculum shaking rate 180 r/min and medium volume 75mL in 250mL flask. The mixed submerge cultivation of T.reesei and Aspergillus niger with a spore ratio of 60∶1 showed that A. niger significantly enhanced the enzyme activity, βglucanase activity was 2.9 times higher than control. Under the optimal conditions,βglucanase activity and FPA activity achieved were 127.6 U/mL and 84.8 U/mL,respectively更多还原