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四磺基铜酞菁共振光散射法测定人血清白蛋白
Resonance Light Scattering Method for Determination of Human Serum Albumin with Cu4S4Pc
【摘要】 研究了四磺基铜酞菁与人血清白蛋白作用的共振光散射光谱 .p H=1.0~ 3.0的范围内 ,人血清白蛋白的加入导致四磺基铜酞菁共振散射的增强 .在 4 0 7nm处存在一共振光散射强峰 ,其强度增加值与人血清白蛋白的浓度呈线性关系 ,据此建立了一种用共振光散射光谱测定人血清白蛋白的方法 .该方法的线性范围为 0~ 17mg/ L,检出限为 0 .0 5 0 mg/ L(n=6 ,RSD为 1.8% ) .
【Abstract】 The resonance light scattering (RLS) spectra of thtrasulponaeted copper phthalocyanine (CuS 4Pc) with human serum albumin (HSA) are studied. The RLS of CuS 4Pc is greatly enhanced by HSA in pH range of 1.0~3.0. There is a resonance light scattering peak at 407nm, and the enhanced intensity of RLS at this wavelenght is proportional to the concentration of HSA. So a method of RLS for the determination of HSA is established. The linear range is 0~17 mg/L. The detection limit is 0.050g/L(n=6, RSD is 1.8%). This method is simple and rapid.
【Key words】 thtrasulponaeted copper phthalocyanine; resonance light scattering; human serum albumin;
- 【文献出处】 郑州大学学报(理学版) ,Journal of Zhengzhou University (Natural Science Edition) , 编辑部邮箱 ,2003年01期
- 【分类号】O657.39
- 【被引频次】9
- 【下载频次】78