【摘要】 目的　研究北方汉族人群儿童急性淋巴细胞性白血病 (ALL)患者与HLA A ,B ,DRB1等位基因多态性的遗传关联。方法　采用聚合酶链反应 序列特异性寡核苷酸探针 (PCR SSO)方法 ,对 197例北方汉族儿童ALL患者和 5 84 1例健康脐带血样本HLA A ,B ,DRB1等位基因多态性进行研究。结果　在HLA A ,B ,DRB1等位基因中 ,儿童ALL患者的A11(18.5 3% ,χ2 =8.5 9,P <0 .0 1,RR =1.4 7,EF =0 .0 6 35 )和A2 4 (19.2 9% ,χ2 =11.6 5 ,P <0 .0 1,RR =1.5 6 ,EF =0 .0 738) ;B4 0(18% ,χ2 =1199.6 2 ,P <0 .0 0 1,RR =5 3.2 3,EF =0 .18) ,B15 (30 % ,χ2 =15 9.17,P <0 .0 0 1,RR =9.6 89,EF =0 .0 14 ) ,B5 6 (1.5 % ,χ2 =16 .17,P <0 .0 0 1,RR =5 .92 ,EF =0 .0 774 ) ,B6 7(2 .5 % ,χ2 =5 .93,P <0 .0 1,RR =2 .74 ,EF =0 .0 774 ) ,和B2 7(3.75 % ,χ2 =4 .96 ,P <0 .0 1,RR =1.81,EF =0 .0 774 ) ;DR9(18.2 1% ,χ2 =16 .0 7,P <0 .0 0 1,RR =1.74 ,EF =0 .0 774 ) ,DR12(14 .13% ,χ2 =7.12 ,P <0 .0 1,RR =1.5 2 3,EF =0 .0 4 9) ,DR14 (7.88% ,χ2 =5 .194 ,P <0 .0 5 ,RR =1.5 74 ,EF =0 .0 2 87) ,和DR16 (4.89% ,χ2 =8.5 2 7,P <0 .0 1,RR =2 .0 6 5 ,EF =0 .0 2 5 )等基因的基因频率都显著高于正常人群更多还原

【Abstract】 Objective: To study the genetic susceptibility of HLA?A, B, DRB1 alleles in children patients with Acute Lymphoblastic Leukemia (ALL). Methods: HLA?A, B, DRB1 alleles polymorphism in 197 cases with ALL and 5841 normal umbilical cord blood controls were determined by PCR with sequence specific olignucleotide probe (PCR?SSO). Results: The allele frequencies of A11(18.53%,χ 2=8.59,P<0.01,RR=1.47,EF=0.0635),A24(19.29%,χ 2=11.65,P<0.01,RR=1.56,EF=0.0738),B40(18%,χ 2=1199.62,P<0.001,RR=53.23,EF=0.18),B15(30%,χ 2=159.17,P<0.001,RR=9.689,EF=0.0145),B56(1.5%,χ 2=16.17,P<0.001,RR=5.92,EF=0.0774),B67(2.5%,χ 2=5.93,P<0.01,RR=2.74,EF=0.0774),B27(3.75%,χ 2=4.96,P<0.01,RR=1.81,EF=0.0774); DR9(18.21%,χ 2=16.07,P<0.001,RR=1.74,EF=0.0774), DR12 (14.13%,χ 2=7.12, P<0.01,RR=1.523, EF=0.049), DR14 (7.88%,χ 2=5.194, P<0.05,RR=1.574, EF=0.0287), DR16 (4.89%,χ 2=8.527, P<0.01,RR=2.065,EF=0.025) were increased, while the allele frequencies of HLA?DR7 (8.15%,χ 2=7.078, P<0.01,RR=0.60,PF=0.086),DR15 (12.23%,χ 2=4.049, P<0.05,RR=0.7109, PF=0.067) were decreased in patients with ALL. Conclusion: HLA?A11, A24, B15, B58, B67, B27, DR9, DR12,DR14,DR16 alleles seem to contribute to the genetic susceptibility for children patients with ALL, especially B40 allele, while HLA?B48, DR7, DR15 to its genetic resistance.更多还原