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三个拮抗菌株对辣椒青枯病的作用机制

The Mechanisms of Three Antagonistic Strains Against Bacterial Wilt in Capsicum

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【作者】 郭坚华龚龙英祁红英张立新方中达

【Author】 GUO Jianhua, GONG Longying, QI Hongying, ZHANG Lixin, FANG Zhongda(Department of Plant Protection, Nanjing Agricultural University, Nanjing 210095 , China)

【机构】 南京农业大学植保系南京农业大学产业处江苏淮安市蔬菜研究所南京市栖霞区农技推广站南京农业大学植保系 南京 210095南京 210095

【摘要】 对辣椒青枯病菌生物防治机制的研究中发现,J2菌株(沙雷氏菌)的无抑菌能力突变株只保留了15.5%的防病效果,比野生型J2菌株的防效降低了77.6%,推测其生物防治功能主要依赖于抑菌能力。未检测出其定殖的J3菌株(假单胞菌)突变株,对青枯病的防治效果只有7.7%,比野生型J3菌株下降了90.0%,推测J3菌株主要依赖于在植株根部的定殖竞争能力。分根试验表明,BB11菌株(芽孢杆菌)的防病功能主要依赖于诱导抗性作用,亦有抑菌作用的因素。菌株J2、J3和BB11在灭菌土和自然土中均有良好的定殖表现。

【Abstract】 Mechanisms of three bacterial strains, Serratia sp. J2, Pseudomonas sp. J3 and Bacillus sp. BB11, to control pepper bacterial wilt disease were studied. Results indicated that the biocontrol effectiveness of m utant J2UI-, notinhabitable in vitro against Ralstonia solanacearum , was reduced to 155% from 692% expressed by the parent strain J2, indicatin g that antibioticproduction is an important factor in the suppression of the d isease by J2. The biocontrol effectiveness of mutant J3TC-, which lost its co lonization ability in pepper root, was only 77%, 90.% lower than the effective ness of its parent strain J3, which indicated that the J3 strain is dependent on strong colonization in root system to control the disease. Rootsplitting exp eriment showed that induced systemic resistance was very important for strain BB 11 to control the pepper disease. All the three parent strains could colonize w ell in the root system of pepper.

【基金】 江苏省应用基础(BJ93072);南京市自然基金(20011016)资助项目
  • 【文献出处】 中国生物防治 ,Chinese Journal of Biological Control , 编辑部邮箱 ,2003年01期
  • 【分类号】S436.418
  • 【被引频次】51
  • 【下载频次】443
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