【摘要】 草鱼样品体重(1543±213)g。采用离体消化培养和茚三酮比色方法,以草鱼前肠、中肠、后肠和肝胰脏的粗酶液作为酶源,对鱼粉、豆粕、菜粕和棉粕进行酶解,并测定酶解14h内,豆粕酶解液中氨基酸生成量随时间的变化,以及0至4h,内4种饲料蛋白质酶解液中氨基酸的生成量。结果表明,(1)在0至4h内,4种饲料酶解液OD570随时间变化的线性关系较好。(2)对于同种蛋白质饲料原料,以中肠组织粗酶液的酶解速度最大,其余依次为前肠、后肠及肝胰脏粗酶液。表明草鱼中肠对饲料蛋白质的酶解能力强于前肠和后肠,同时,肠道酶液的酶解速度均大于肝胰脏。(3)对于4种不同的蛋白质饲料原料,以鱼粉酶解氨基酸生成速度最大,其他依次为豆粕、菜粕、棉粕。(4)草鱼肝胰脏粗酶液对豆粕的酶解氨基酸生成速度最大,表明草鱼对豆粕进行酶解消化的能力较强。实验证实,饲料原料种类的差异和饲料蛋白质组成与性质的差异使其酶解氨基酸生成量和生成速度有差异。更多还原

【Abstract】 The digestive enzymes for the enzymolysis cultivation were extracted from the anti intestine, middle intestine, post intestine and the hepatopancreas of grass carp, respectively. The fishmeal, soybean meal, rapeseed meal and cottonseed meal were used to be digested by those digestive enzymes at 28 ℃, and the amount of amino acids produced in the enzymolysis fluid were tested within 0 h to 4 h. The results show that: (1)There is a linear relationship between the amino acids production amount and the digestive time. (2)For the same raw material protein, the fastest digestive rate occurs in the enzymolysis fluid from middle intestine and followed the enzymolysis fluid from anti intestine, post intestine and hepatopancreas.Meanwhile,the enzymolysis fluid from intestine has higher digestive rate than that from hepatopancreas. (3)For the four raw feed, the aminoacidsproducing rate is in the order of fish meal>soybean meal>rapeseed meal>cottonseed meal. (4)The digestibility in the intestine is higher than that in the hepatopancreas.更多还原