【摘要】 目的　在大肠杆菌中表达登革热病毒外膜抗原基因 ,获得能作为检测抗原的重组蛋白。方法　登革热 2型病毒NGC株感染C6/ 36细胞后 ,抽提病毒RNA ,经逆转录和套式PCR扩增出E基因片断 ,扩增片段经酶切、连接、克隆 ,构建重组表达质粒。并转化大肠杆菌DH5α。结果　重组质粒经诱导后表达外膜抗原的部分肽链。经过免疫印迹证实其具有免疫反应性。结论　截断的部分E蛋白基因在大肠杆菌中表达成功 ,重组的蛋白可作为血清学检测抗原使用更多还原

【Abstract】 Aim To obtain the recombinant antigen used for detection of dengue virus infection Mothed The Dengue virus NGC strain,serotype 2 virus,was cultured through infected the C6/36 monolayer cell The viral RNA was extracted from the cultured supernatant The artificial synthesized primers were applied to amplify the viral envelope antigenic gene fragment by reverse transcription nested PCR The PCR amplified fragment with restrict enzyme terminus was inserted the vector to construct the recombinant expression plasmid Result The recombinant polypeptide was yielded in E coli by induced with IPTG Western blotting indicated the recombinant polypeptide Could be recognized and combined by the serum against the dengue virus from the patient infected with the virus Conclusion The truncated envelope antigenic gene has been successfully expressed in E colias a antigen for dengue serum detection更多还原