【摘要】 选择 40只 3 0日龄雌性大鼠分为 4组 ,分别用含重组抑制素α(1～ 3 2 )基因真核表达质粒pcINH 0 μg(对照组 )、15μg(试验组 1)、2 5μg(试验组 2 )、40 μg(试验组 3 )的脂质体进行主动免疫 ,间隔 2 0d后用不含脂质体的重组基因质粒加强免疫 1次。结果发现 ,抗体阳性鼠占 50 % (13 / 2 6) ,但加强免疫 10d后的抗体P/N值没有升高 ,而且免疫剂量的增加并没有提高阳性鼠的比例 ;抗体阳性鼠卵巢上的成熟卵泡数比阴性鼠多 2 .3 ,但统计差异不显著 (P >0 .0 5)。首次免疫 10d后阳性鼠血浆FSH浓度显著升高 (P <0 .0 5) ;但血浆雌二醇水平无显著差异 (P >0 .0 5)。加强免疫 10d后血中促卵泡素水平没有升高。本试验结果证明 ,应用重组抑制素真核表达质粒免疫动物可以产生抗抑制素抗体。更多还原

【Abstract】 A total of 40 rats at the age of 30 days were divided i nto 4 groups and immunized (intramuscularly injection) with 0 μg(control)?15 μg (trial 1), 25 μg (trial 2) or 40 μg(trial 3) of inhibinα(1-32) recomb inant expression plasmid pcINH in combination with liposome. Booster was given w ithout liposome on day 20 after primary immunization. The results showed that 50 %(13/26) of rats were detected in positive antibody against inhibin. However, th e increase of immunization dosage and booster did not promote the ratio of antib ody positive rats. The number of matured follicles above 0.8mm in diameter in th e antibody positive rats was 2.3 more than that in the negative rats (P>0.05 ). The concentration of blood plasma FSH increased distinctively 10 days after p rimary immunization (P<0.05), but no increase was observed after booster imm unization. The 17β-estradiol levels in blood plasma of rats between the positi ve and the negative groups had no remarkable differences (P>0.05). These result s suggested that recombinant inhibin expression plasmid can stimulate animal bod y to produce antibody against inhibin.更多还原